COUNTER CURRENT CHROMATOGRAPHY: QUATTRO CCC™ / HPL-LC™ REVOLUTIONARY, GREEN, PREPARATIVE & PROCESS TECHNOLOGIES

 

Our page insertion in issues of LCGC Journal

   

             Labprep


PLEASE CLICK ON ONE OF THE FOLLOWING UNDERLINED TO GO TO OUR NEW WEBSITE
www.quattroprep.com for counter current chromatography , countercurrent chromatography and centrifugal partition chromatography as this website is presently being redesigned.

If you face a challenge with micro, laboratory or process scale of solid-liquid chromatography / extraction, including Flash Chromatography, HPLC, HPLC MS, SFC or SFC MS, SFE you should contact us now. Is your challenge on-column adsorption or on-column degradation? Are your Process Flash / HPLC / SFC / SFE phase costs / solvent costs too high? Do you have unexplained loss of bioactivity? All above problems can be solved with Liquid-Liquid Chromatography / Extraction solutions unique to us, using our just released modular, Quattro Mk 7, High Pressure Liquid-Liquid Chromatography™ / Extraction™ (HPL-LC™/HPL-LE™). Please read our Liquid-Liquid Chromatography / CCC / CPC Review Article, Click Here

We have over 18 years of Liquid-Liquid Chromatography / Extraction experience creatively solving both academic and commercial research problems. We have evolved far past historic Counter Current / Centrifugal Partition Chromatography (Hydrodynamic and Hydrostatic CCC / HSCCC / HPCCC). Historic HSCCC / HPCCC work at 105 psi. Quattro HPL-LC™ works at over 1000 psi and offers you new possibilities in HPLC MS, SFC MS & HPLC / SFC / SFE Process Chromatography etc. For an introduction slide show please click 800 or 1024+

 

For Brochures, Please
Click Below To Enlarge


QuikPrep™ Page 1

QuikPrep™ Page 2

LabPrep™ Page 1

LabPrep™ Page 2

PrepPlus™ Page 1

PrepPlus™ Page 2

ProcessPrep™ Page 1

ProcessPrep™ Page 2

New Products

Please Click Any Button Or Underlined Title To Link to Appropriate Page
  
New LabPrep MK5

Flash Chromatography

Home 

Why use CCC
CCC complimentary HPLC

Retail Prices
CCC Intro
Company Intro
Publications
Rio CCC 2001

Please Click Either The
Underlined Title Or The
Thumbnail For A CCC
Applications Slide Show
Lecture

Sequential CCC-HPLC

CCC of API Glycosides



General CCC Applications

CCC

CCC of API Antioxidants

CCC


UNDERSTANDING QUATTRO CCC OPERATION VIA A VIDEO : CLICK LINK

SUMMARY OF QUATTRO CCC COUNTERCURRENT MODEL RANGE & DIMENSIONS

NEW QUATTRO CCC™ APPLICATIONS SLIDE SHOWS
NEEM 800 or 1024+ , WINE RESEACH 800 or 1024+


Special Installation and Training Packages where we spend one week in your lab, working on your target compounds by our novel, generic Sequential CCC-HPLC methodology which was developed by us over our 15+ years of combining CCC & HPLC.

Please view the video, plus the various slide shows, above and at bottom of left side bar for rapid, easy to understand, pictorial introduction to counter current chromatography and research grade science on Quattro CCC applications. Many of these slide shows were presented at International Symposia either by Quattro CCC users or ourselves. Click on any of the brochures in left bar to make full-screen for Quattro CCC additional technical specification information, and details of our modular, model range.

Introducing AECS-QuikPrep Ltd

First established in 1983 as AECS, we recently renamed to AECS-QuikPrep Ltd. We are after 25 years of trading, one of the longest established chromatography instrument design and production manufactures in the United Kingdom. Our Managing Director and co-founder, Dr Les Brown is still a practising chromatographer and has personally been involved in countercurrent instrumentation sales, design and the development of generic counter current / liquid-liquid chromatography method development strategies since the early 1990’s.

    Dr L Brown             
              Dr Les Brown                           

Les still enjoys working in the lab on challenging research, either for contract chromatography method development projects, or custom purification of known targets and unknown bio-actives, from natural products and synthetic research projects. Les & others at AECS personally visit clients worldwide to train researchers in CCC or CPC or HPLC generic method development strategies. Mr Robin Self our

Why not contact us about your needs? We are like-minded researchers and instrumentation engineers, who intimately understand the fundamentals of both solid-liquid chromatography and liquid-liquid chromatography.

For Contact Details Please Click Here

Introducing Quattro CCC and explaining what they can do to benefit you

   GI & SU

Quattro CCC™ are fun! Prof Dra Gilda & Suzana Guimaraes Leitao, Joint Chairpersons of CCC2008, Rio, Brazil, celebrating the arrival of their Quattro HTPrep™ prior to installation in their laboratory. This HTPrep™ was used for the Seminar's Workshop on Liquid-Liquid Chromatography

The NEW HTPREP AND INTROPREP HT QUATTRO CCC Mk 6’s can both be used for rapid prep of small scale (sub mg to 100+ mg) and larger mass (100+ mg to multi g) resolution on a single Quattro CCC as they have at least two coils of different volumes and different id’s (typically a high resolution / low mass injection ~20 ml coil of 1.0 mm id and a higher mass injection ~100ml coil of 2.0 mm id). Both these instruments are also ideal for ccc-MS.

IntroPrep IntroPrep  IntroPrep

At CCC2008 the HTPrep purchased by the Federal University of Rio (shown above) was utlised to demonstrate CCC / CPC method principles to attendees of CCC2008 at the CCC / CPC practical workshop sessions. Already several attendees are moving to purchase. Why not join them and the many others throughout the world who use Quattro CCC.

Uniquely we will CUSTOM DESIGN Quattro CCC / Prep HPLC for specific client needs. Do you have a novel chromatography requirement, why not come talk to us?

Our New HTPrep and IntroPrep HT were designed to meet the needs of  Major Pharmaceutical Company to exploit liquid-liquid chromatography in their High-Throughput Purification Group. We believe the first ever application of countercurrent chromatography for routine HT was given at CCC2008 by their staff after their novel HT research on their Quattro CCC HTPrep. This oral lecture will shortly be published in CCC2008 proceedings.

             CCC Silver           CCC Silver

Shown above is an HTPrep with full automation capable of interaction with external computer systems, for integrated HT. This is the Quattro CCC presently used by the Major Pharmaceutical Company, which utilises their design of computer programme to control sample injection, HPLC pumping, Quattro CCC HTPrep, detector, fraction collection, and data system. Additional features to standard for this HTPrep included automation, solvent leak sensor, vibration sensor and bench lock-down.

Les & others will travel anywhere in the world to assist in CCC / CPC / HPLC research projects (see also our HPLC website www.chiral-hplc.com ). A few recent examples of training courses / co-research projects are Rio, Brasil 2001, Vicosa, Brasil 2004, Adelaide, Australia 2006, Chicago, USA, 2007, Guangzhou, China 2007 etc. We assisted in founding the bi-annual CCC200X series, which started in London in 2000, and the Rio-CCC2001 series. We were also the first commercial exhibitor sponsor for CCC2006 at NIH, USA, where we exhibited. We sponsored CCC2008 in Rio, Brasil and have been appointed a co-supporter for CCC2010 in Lyon. AECS-QuikPrep Ltd is a TRULY INTERNATIONAL COMPANY, which believes and lives by its belief, that science is a totally international enterprise.                       


Since the early 1980’s AECS-QuikPrep has been evolving its HPLC products, and from early 1990’s its counter current chromatography products into the preparative chromatography instruments / consumables of first choice for laboratory, pilot and process plant preparative scale applications

QUATTRO CCC INSTRUMENT TYPE DEFINITIONS

The new, low-cost IntroPrep series consists of the introductory Quattro CCC chassis option, and is offered with two bobbin pack options, one with a single coil, the other with a 2 coil options. Only PTFE coils are available on the IntroPrep series.

          Quattro Mk 5             Quattro Mk5 

The QuikPrep™ high performance liquid-liquid chromatograph™ (HPL-LC™), introduces both PTFE and Stainless Steel coil options to countercurrent chromatography. QuikPrep offer enormous choice of coil options from 20 ml with 1 mm id coils up to ~750 + ml total capacity with large id’s. The former could be used for sub milligram preps and the later for preps up to 30 + g injected mass. Yet all this is possible within a minimally sized chassis (see pictures).  The QuikPrep has 2 bobbins per instrument and the option of either 2 or 4 coils per instrument. Coils of same id can be used in series or in parallel. All four coils could be doing different preps at the same time if required. Chose your chassis. Chose chassis options. Chose coil id and material. Chose number of coils. You design your Quattro CCC to fit your requirements. We build to your specifications (see brochures for options, link via thumbnails on left vertical bar).

The LabPrep approximately doubles the volume capacity options available on a QuikPrep. Shown below is a LabPrep CCC in a typical user system, inclusive of HPLC pump, injector and detector. Think of a Quattro CCC as a mechanical Prep HPLC column, and fit it into your HPLC system as you would a Prep HPLC column.

LabPrep

As an Quattro CCC often uses a third to a tenth of the solvent that might be used by a Flash or Prep HPLC for the same mass injected, standard analytical HPLC pumps, which pump up to 10 ml / min, may be utilised with a LabPrep being used for preparing multiple 10’s of grams of target containing matrix injected. This represents a massive solvent saving and has the convenience of utilising standard analytical HPLC pumps, which most labs have available. This saves the cost of buying dedicated Prep HPLC pumps, which would definitely be required even for 21 x 250 mm Prep HPLC columns. A 21 x 250 mm HPLC in reverse phase material with a typical flow requirement of 20 ml / min, would be expensive to purchase. A single contaminated sample might destroy it, plus it would only be capable of “on-column” loading of 100 to 1000 mg, rather than the 10’s g loading of a Quattro CCC utilising 10 ml / min flow rates. To achieve 1’s or 10’s grams injected, prep or process HPLC flows of 50 to 1000 ml / min might be required. Hardly any wonder we recommend the AECS designed Sequential CCC-HPLC generic gradient procedures. Inject crude extract on to a Quattro CCC, run a generic gradient. Each CCC fraction would have such a small polarity range that an isocratic HPLC run would be suitable, thereby massively reducing the risk of HPLC column contamination. Plus the target to contaminant mass in the Quattro CCC derived fraction would probably reflect a 50 to 95+ % target purity already by Quattro CCC. If insufficient purity was achieved by Quattro CCC alone, the relatively low mass / high purity of the CCC fraction would then make, sequential, final prep HPLC very cost-effective, with low risk of HPLC column contamination. Why not contact us to discuss your needs?

  HPLC    HPLC    FLASH

THE QUATTRO CCC IS PROBABLY THE ULTIMATE IN PREP CHROMATOGRAPHY WHICH IS BOTH A GREEN, PLUS A COST EFFECTIVE TECHNOLOGY. IT USES MINIMAL SOLVENT. NO EXPENSIVE STATIONARY PHASE TO BUY OR TO WORRY ABOUT WITH RESPECT TO CONTAMINATION. INJECT CRUDE, FILTERED, DISSOLVED EXTRACTS, DO ONE OF OUR GENERIC POLARITY GRADIENTS. THEN USE WITH OUR QUIKPREP HPLC COLUMNS TO OBTAIN PURE TARGET, BY SEQUENTIAL CCC-HPLC…….IT’S A NO-BRAINER…..ITS SO EASY !!!

A PrepPlus approximately doubles the capacity options available on a LabPrep

                                PrepPlus

ProcessPrep are custom built in any volume option up until 100’s of litres capacity.

                                 ProcessPrep

For the most recent eight years over 85% of our sales have been overseas, with Quattro CCC instrument sales, plus instrument design, & preparative chromatography / technical & market research consultancies in Australia, Benelux, Brazil, Canada, Chile, China, France, Germany, Singapore, Sweden, Switzerland, USA etc.

We are one of the longest established liquid-liquid chromatograph specialists in the world. Recessions have come, and recessions have gone in last 25 years, AECS is still here and will still be here for you a long time in the future. We have always been self-financing. We have existed for over 25 years based on commercial and profitable trading. Certain of our much more recent CCC competitors exist mainly by Venture Capital & Bank Loan cash injection support and collaborative Grants with an associated University, rather than by profit income. Neither Venture Capital nor Grants are stable or long-term, especially in the present World Economic climate. We have over 25 years of proven stability, please chose your instrument supplier wisely, and come to discuss your needs with us.

Quattro CCC Instrument Design History

   Quattro Mk 1   Quattro Mk 2

Mk I Quattro CCC (early 1990’s) Mk II & Mk III Quattro CCC mid 1990 to 2002

From the early 1990’s to 2000 we utilised standard instrument design techniques for our Quattro CCC. Feedback from customers showed us this was inappropriate in liquid-liquid chromatography, so we assigned this technology to Brunel University, UK, subject to a fee being paid and certain conditions. We collaborated with BIB / Brunel University in CCC research projects from 1993 to 2000, but then chose to cease all future collaboration as they became competitors in 2000, then more recently, shareholders in a start-up UK Ltd that Brunel University and it’s Professor/staff co-founded. Other collaborations have included, AstraZeneca, GSK, Shell Research, Syngenta (then AZ Agrochemicals), University College of Swansea and University College of London.

Based on our own internal research we developed “in-house” a new MODULAR CONCEPT for the Quattro CCC Mk IV, which was released in 2002. From 2002 all Quattro CCC now offer our unique modularity. All Quattro CCC from the 20 ml ccc-MSPrep coil to a 100+ litres ProcessPrep share the same planet & sun radii, beta value ranges, speed range options etc. This design concept was further updated to the Quattro Mk 5 design in 2005.

       Quattro Mk 4       Quattro Mk 5   

          Style of Mk IV Quattro CCC                         Style of Mk 5 Quattro CCC

In 2007 we began sales of our new QUATTRO CCC™ Mk 6 range, and the HTPrep™ (shown earlier in this page).

In 2008 we introduced the ccc-MSPrep™ & IntroPrep™

We have a lot of CCC & HPLC experience; why not discuss your needs with us?

As you can see, we have been involved in a progressive upgrading of CCC design for over 15 years. If you are interested in High Throughput / Combinatorial Chemistry, micro scale prep of synthetic and/or natural products, or ccc-MS, or lab / process scale chromatography, and need to talk to long-term experts on liquid-liquid chromatography, please contact us for more details of what Quattro CCC™ liquid – liquid chromatography and sequential CCC-HPLC could do for you.

For Contact Details Please Click Here

Unlike conventional high pressure liquid chromatography (HPLC), low pressure liquid chromatography and flash chromatography, which are all solid-liquid chromatography systems, SLC ……… With countercurrent chromatography, the chromatographic process utilises one immiscible, immobilised, stationary liquid, and another immiscible, flowing mobile liquid, thus a more accurate name than counter current chromatography would be, high performance liquid-liquid chromatography, HPL-LC™.

To summarise, one of the pair of immiscible, biphasic liquids is immobilised in a planetary centrifuge of a Quattro CCC™ design, and the other flows over this, periodically mixing with and separating from the stationary liquid

Imagine taking a separating funnel and doing 100,000 separations in an hour……….a Quattro CCC™ does the equivalent of this.

Counter-current chromatography can separate diasteromers, chiral & non-chiral API’s, natural products, synthesized compounds, petrochemical, inorganic anions and cations, precious metals and radio active compounds without the risk of “on-column degradation" or "infinite adsorption".


The Quattro CCC™ range has been designed as a modular system, which allows predictable scale up from sub-milligram on a minimum footprint laboratory instrument, to a multi-rotor simulated moving bed countercurrent chromatography multiple tonne per annum system, designed to work in a process, intrinsically safe environment.  
      
The major benefits of counter-current chromatography are : “No Infinite Adsorption”; “No On Column Degradation", ”No Need For Expensive Stationary Chromatographic Phase”, “Can Be Inorganic and/or Organic; The Inorganic can be Metal, Non Metal or Radionucleotides; The Organic can be, Chiral or non-Chiral, Reverse or Normal Phase, Miscelle or Reverse Miscelle, Liquid Ion Exchange", “From Infinity Polar or Infinity Non Polar”, “One Quattro CCC Could Be Performing Up To 4 Separate Preparations”, “Can Prepare From Crude Extract Narrow Polarity Ranges Suitable For Prep HPLC Without Fear Of Contaminating The HPLC Column” and often a “Reduction Of 3 To 10 Times In The Solvent Usage, As Compared To Preparative Flash Or HPLC”.

Traditional, sub-critical (HFC’s) and super critical fluid (CO2) solvents may all be utilized in chiral & non-chiral counter current chromatography.

Web based “Tele-Lecture” courses are available at no charge, to assist you in both understanding countercurrent chromatography and in choosing the appropriate counter current chromatography instrumentation for your needs.

 
IN OCTOBER 2008, AECS CELEBRATED 25 YEARS OF : 

1. COMMERCIAL ANALYTICAL, PREPARATIVE AND PROCESS, CHIRAL & NON-CHIRAL CONTRACT RESEARCH METHODOLOGY DEVELOPMENT,



2. PLUS CONTRACT INSTRUMENTATION DESIGN.

AECS HAS BEEN SUPPLYING VARIOUS MODELS OF THE QUATTRO CCC™ RANGE, INTERNATIONALLY SINCE 1995.

ALL MODULAR QUATTRO Mk IVs & Mk 5/6's ARE OF AECS DESIGN AND BUILD.

ALL MODELS OF CCC OF ANY COMPANIES MANUFACTURE CAN BE CUSTOM UPDATED BY AECS-QUIKPREP™.


 LINK FOR OUR CHIRAL & NON-CHIRAL ANALYTICAL / PREP / PROCESS HPLC COLUMNS & CONTRACT PREP



For Contact Details Please Click Here

 

 

 

 

 

 

 

 

                                                    

__