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CCC4LABPREP FOR THE QUATTRO CCC™ AND ADVANCED COUNTER CURRENT CHROMATOGRAPHY     



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HISTORIC QUATTRO
CCC™ s

      







Slide 1…..( Lab Prep CCC™ …..Unit open )

The Quattro CCC™ LabPrep 710 dimensions are 710 mm x 520 mm x 500 mm. The Bobbins vary in weight depending on coil tubing material ( PTFE or Stainless Steel ) plus the coil length / volume and the ID & OD of the tubing. An approximate rule in Counter Current Chromatography is an injection mass of 1 g per 100ml volume for a 1.6 mm bore with the Beta Values of a Quattro CCC™.

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The actual mass injected will depend on solvency in stationary and mobile phase plus the resolution requirement. A range of 0.5 to 2 g per 100ml would cover many applications.

Any coil can be used with a near infinite number of biphasic Counter Current Chromatography solvent options.

A 100 ml coil when preparing via a step gradient, bio-actives from the wide dynamic range of polarities that might be present in a complex natural product, would typically require …………100 ml to fill coil with stationary phase, ……….10 to 30 ml to fill coil. ( once coil is rotated ) with mobile phase, then for a simple two step gradient, ………10 to 30 ml each of two chosen biphasic mobile phase mixtures,………. and finally another 100ml to flush the coil of both stationary and mobile phase, once rotation has been stopped.

Thus the solvent usage to prepare a complete polarity scan from infinitely polar to infinitely non-polar unknowns in a complex natural product mixture would require approximately 260 ml for approximately 1 gram injected mass. The flows would typically be in the 2 to 10 ml / min range and therefore available at no additional expense from a standard analytical HPLC solvent pump.

This solvent usage compares very favourably with Preparative HPLC and Flash Chromatography.

In addition, Counter Current Chromatography has the great advantage of no risk of irreversible adsorption onto the solid phase, no risk of degradation on the solid phase and in the case of expensive preparative HPLC columns, no risk ( precipitation aside ) of ruining the column by contamination.



                 


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