This slide shows the TLC results from the partitioning test. For each solvent A, B, C, D, and E two spots are applied :- one for the organic phase (S = superior) and one for the aqueous phase (I= inferior).

This tlc plate shows that as the amount of methanol increases in the systems, more compounds are extracted from the organic phase into the aqueous phase, which is to be the MOBILE PHASE. More polar compounds will thus elute first in the proposed reverse phase CCC chromatograph that will utilize the aqueous phase as the mobile phase. The less polar a compound the longer it will be retained. Infinitely non-polar compounds will not elute during the active gradient but will be eluted when the rotation is switched off and both the mobile and stationary phase in the column are then washed out together as the Archimedean Principle which previously retained the stationary phase is inactivated. Little band broadening occurs during washing out and the resolution of compounds in the coil is preserved. The stationary phase infinitely retained compounds will elute in the last drops of stationary phase washed out of the column.

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