The shown tlc plate summarizes the results obtained in this experiment. The first spot refers to the total extract for comparison.

As discussed above, the more polar compounds elute first, leaving the less polar ones to elute later. It can be seen from the results, that the gradient system utilized allowed the separation of two alkaloid derivatives, from the multitude of other extracted components present. These compounds are selectively visualized by the development with Dragendorff reagent. Subsequent MS/NMR etc analysis showed that one of the components was in a highly pure form in the fraction from the CCC. It's important to point out that these two compounds were not separated when using solid / liquid chromatography preparative techniques

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