Fractionation of the CH2Cl2 extract by traditional chromatographic methods, such as silica gel open column chromatography combined with preparative reversed phase HPLC, failed.

About 40% of the crude extract binds irreversibly to the silica, which means that potentially a significant loss of important bioactive substance could occur. Counter-Current Chromatography is a technique were all components injected can be recovered and which only exposes compounds to aqueous / organic solvents and is thus less likely to destroy / irreversibly bond; labile / adsorptive compounds than would prep tlc, open tubular, hplc or flash chromatography techniques. Thus CCC was investigated after the failure of solid / liquid chromatographic techniques tested.

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