Non-Chlorosilane Bonding and Triple End-Capping Make QuikPrep Enhanced™
HPLC Phases and Columns So Special !!!
Columns, columns, columns… Plus, everyone claims to have
the best column for your application. It comes down to four main factors:
low silanols, low trace metals, high efficiency and excellent symmetries.
Similar high quality results are obtained for our 4µm, 100Å QuikPrep Enhanced™ RR columns, with p/m of 95,000 to 110,000 being standard. Yet, both are as competitively priced as any quality 5µm HPLC column.
Can your 5µm, C18 or C8, 100Å 4.6x150mm
column give these results?
How pH stable are our columns compared to other brands?
Sure, all our columns are stable to pH 1.5 – 8.5. What about any
application specific results? Single columns which utilized our unique
non-chlorosilane bonding of phase and end-capping have been used for
specific peptide analyses for over one year, with the column continuously
exposed to flowing or stationary 0.1% TFA, 49.95% DW, 49.95% Acetonitrile
throughout the year, without significant loss of performance.
This is why our QuikPrep Enhanced™ are so special. We do not use chlorosilanes for phase or end capping. Plus, we use one ethyl dimethyl and two trimethyl end capping stages while washing each phase copiously with each bonding step.
In addition, most column manufacturers utilize high purity solvents during bonding, end capping and washing of each phase. We go to the extreme in order to provide a superior product. Each solvent used starts as an ultra high purity solvent and is then glass distilled internally to remove additional metals and contaminants that will negatively affect our final product. Silica, as you know, is like a sponge. So, while most column manufacturers offer a metal-free silica as their starting material, what’s their final product’s metal content?
If you are considering a semi-prep or prep HPLC column, consider our ultra high quality line. Beyond what we have mentioned above as resulting in a superior product, we offer our standard analytical grade material in 4 or 5µm exactly the same as the analytical size columns. Many column manufacturers reduce the selling price of their semi-prep and prep columns by offering larger particle size (8µm & up). You might say, I am already aware of this. But, did you know that when they offer it in a 5µm analytical grade, often times, it is not the same particle size distribution? Our particle sizing is selected based on 90/10 size distribution whether in analytical size columns or semi-prep and prep size. Many competitors use an 90/10 for 5µm analytical grade but 80/20 distribution 5µm prep grades.This greatly reduces the their final prep column cost of their product, but also their efficiency.
How would you know the size distribution, simple, have you tried to scale up a 4.6 mm id to 10 mm id or 21 mm id in supposedly an analytical grade 5µm and failed? The most likely cause is the NON-analytical grade particle size distribution of your supposedly analytical grade 5µm phase in our competitor’s semi-prep HPLC column.
So how would you check this? First ask them specifically
about particle size distribution.
If our competitors columns are high efficiency at 4.6 mm id and significantly lower with the same supposed phase at semi-prep? We leave you to draw your own conclusions?
consider the advantage of our bonding and triple end-capping on column
washing with redistilled solvent. By definition, if we wash four times,
once each after bonding and three end-capping stages and our competitors’
wash twice, once after bonding and once after end-capping, would you
expect our column bleed to be less? Would you expect our silanol activity
to be less? Would you expect our pH stability within our stated range
to be more? Would you expect our performance to exceed your competitors
Web Site : www.ccc4labprep.com