Why Non-Chlorosilane Bonding and Triple End-Capping Make QuikPrep Enhanced™ HPLC Phases and Columns So Special !!!


Columns, columns, columns… Plus, everyone claims to have the best column for your application. It comes down to four main factors: low silanols, low trace metals, high efficiency and excellent symmetries.

Starting with an ultra-pure, metal-free, spherical type B silica is just the beginning – a common starting point for all column manufacturers. But, that’s where the similarities stop for us. We utilize an unique form of proprietary triple end-capping and non-chlorosilane bonding chemistries along with trace-metal level solvents at each step. These unique steps results in an enhanced HPLC packing and column product line that meets all of the main criteria.

Can your 5µm, C8, 300Å 4.6x150mm column give these results?

The HPLC chromatograph shows a typical Test Chromatograph for the QuikPrep Enhanced™ RR phase packed into our high quality column hardware (we only utilize IT Inc’s highly internally polished columns, for our Enhanced™ RR range for optimum, reproducible results).

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We are not aware of any competitor’s 5µm, 300Å columns which equal the performance above. Can your present column equal the above….?

Similar high quality results are obtained for our 4µm, 100Å QuikPrep Enhanced™ RR columns, with p/m of 95,000 to 110,000 being standard. Yet, both are as competitively priced as any quality 5µm HPLC column.

Can your 5µm, C18 or C8, 100Å 4.6x150mm column give these results?

Our unique bonding and triple end-capping has been optimized for peptides, proteins, LC-MS and difficult to analysis AI’s. Why not test them for yourself?

How pH stable are our columns compared to other brands? Sure, all our columns are stable to pH 1.5 – 8.5. What about any application specific results? Single columns which utilized our unique non-chlorosilane bonding of phase and end-capping have been used for specific peptide analyses for over one year, with the column continuously exposed to flowing or stationary 0.1% TFA, 49.95% DW, 49.95% Acetonitrile throughout the year, without significant loss of performance.

Why do most of our competitor’s use chlorosilane bonding? As a chemist, does it make sense to you to end cap silanols with a trimethyl, etc. chlorosilane, which releases HCl directly at the silica’s surface? OK, you can use techniques to lessen the impact of this HCl. But surely, you would agree with us. It is better not to have HCl released on the silica surface when you bond or end cap?

This is why our QuikPrep Enhanced™ are so special. We do not use chlorosilanes for phase or end capping. Plus, we use one ethyl dimethyl and two trimethyl end capping stages while washing each phase copiously with each bonding step.

In addition, most column manufacturers utilize high purity solvents during bonding, end capping and washing of each phase. We go to the extreme in order to provide a superior product. Each solvent used starts as an ultra high purity solvent and is then glass distilled internally to remove additional metals and contaminants that will negatively affect our final product. Silica, as you know, is like a sponge. So, while most column manufacturers offer a metal-free silica as their starting material, what’s their final product’s metal content?

If you are considering a semi-prep or prep HPLC column, consider our ultra high quality line. Beyond what we have mentioned above as resulting in a superior product, we offer our standard analytical grade material in 4 or 5µm exactly the same as the analytical size columns. Many column manufacturers reduce the selling price of their semi-prep and prep columns by offering larger particle size (8µm & up). You might say, I am already aware of this. But, did you know that when they offer it in a 5µm analytical grade, often times, it is not the same particle size distribution? Our particle sizing is selected based on 90/10 size distribution whether in analytical size columns or semi-prep and prep size. Many competitors use an 90/10 for 5µm analytical grade but  80/20 distribution 5µm prep grades.This greatly reduces the their final prep column cost of their product, but also their efficiency.

How would you know the size distribution, simple, have you tried to scale up a 4.6 mm id to 10 mm id or 21 mm id in supposedly an analytical grade 5µm and failed? The most likely cause is the NON-analytical grade particle size distribution of your supposedly analytical grade 5µm phase in our competitor’s semi-prep HPLC column.

So how would you check this? First ask them specifically about particle size distribution.
Next look at your test chromatographs for 4.6 mm id and your semi-prep columns. Our 5µm, 300Å QuikPrep Enhanced™ RR columns are specified at 85,000 to 105,000 p/m from 2.1 mm id to 21 mm id, our 4µm, 100Å QuikPrep Enhanced™ RR columns are specified at 95,000 to 110,000 p/m and are the same specification again from 2.1 to 21 mm id.

If our competitors columns are high efficiency at 4.6 mm id and significantly lower with the same supposed phase at semi-prep? We leave you to draw your own conclusions?

Now consider the advantage of our bonding and triple end-capping on column washing with redistilled solvent. By definition, if we wash four times, once each after bonding and three end-capping stages and our competitors’ wash twice, once after bonding and once after end-capping, would you expect our column bleed to be less? Would you expect our silanol activity to be less? Would you expect our pH stability within our stated range to be more? Would you expect our performance to exceed your competitors column?

Remember what we suggested makes an enhanced HPLC column? We think we did when we synthesized our Enhanced™ HPLC phases and choose to pack our columns in the highest quality hardware we could find. But, don’t take our word for it that these columns are special. Why not try one for yourself?

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QuikPrep Enhanced™ HPLC

P O Box 80,  Bridgend
S. Wales, UK
CF31 4XZ

TEL : UK (+44) 1656 782 985                                              FAX : UK (+44) 1656 789 282

Web Site : www.ccc4labprep.com