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 Slide 6   shows the loading / resolution of isocratic preparations on a PC Inc CCC ( now obsolete ) with 1.68 mm ID coils of 56 and 315 ml volume which suggests a limit of 400 mg per 315 ml volume in isocratic mode.

Dr Andrew Marston of the University of Lausanne ( Lecture at Rio 2001, Home Page ) has achieve compound loadings of as high as 20 grams on a 600 ml Quattro CCC. The author has found that approximate loadings of 1 gram per 100 ml are typical for Quattro CCC fitted with 1.6 mm ID tubing when used in gradient mode. The actual loading resolvable by HSCCC is both sample and solvent dependent and can hence vary considerably.

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