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 The considerable effectiveness of an appropriate linear gradient can be see by comparing the complexity of the gradient hplc bioactive natural product extract,  Slide 23  to the extremely high purity of the Quattro CCC purified bioactive ingredient shown it  Slide 24.  This slide also shows that 95% of this active ingredient was contained in a single 4 ml fraction which from the UV trace of the CCC run   Slide 22 appeared to be identical to the previous fraction, which in fact contained only a minor trace of this bioactive component.

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