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Whilst linear gradients have been successfully used in many applications including one step isolation of novel bio actives from untreated plant extracts as shown in  Slide 22  a difficulty arises when trying to extrapolate from the linear gradient elution position to a suitable isocratic elution system.
The considerable effectiveness of an appropriate linear gradient can be see by comparing the complexity of the gradient hplc bioactive natural product extract,  Slide 23  to the extremely high purity of the Quattro CCC purified bioactive ingredient shown it  Slide 24.  This slide also shows that 95% of this active ingredient was contained in a single 4 ml fraction which from the UV trace of the CCC run   Slide 22 appeared to be identical to the previous fraction, which in fact contained only a minor trace of this bioactive component.

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