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In gradient HSCCC it is possible to vary either the upper or lower phase modifier solvent in either a normal or reverse phase gradient.

Experimentation has shown in Section B gradient compositions Slide 15 that for reverse phase gradients the best results are usually obtained by varying the methanol ratio and in normal phase gradients the best results are obtained by varying the ethyl acetate ratio.    

The radically different selectivity when doing the above whilst screening complex natural product samples, for novel bioactivity etc. sample is shown in  Slide 21.

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