The text relative to this slide is shown below this slide or click slide to return to main text.

 For this reason it is often easier to ignore test tube simulating a CCC response and instead use a mid-polarity generic CCC gradient as shown in the first lecture presentation :- that is a reverse phase gradient with stepped increases of the methanol content of the aqueous mobile phase used to first define the components polarity, and then begin optimization from the result, as required :-See  Slide 17  and  Slide 18.

Click For Top Of Page