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Section A :- Highly-polar components can usually be accommodated by :-

PEG : Water or Butanol : Methanol : Water gradients.

Section B :- Mid-polar compounds by :-

Hexane : Ethyl Acetate : Methanol : Water gradients.

Section C :- Non-polar components by non aqueous mixes such as :-

Hexane : Modifier : Methanol ( and / or Acetonitrile ) gradients.

Optimization within a Section can often be facilitated by using simple test tube shake partition tests ( with suitable analysis as appropriate, the partition / the relative concentrations in the upper and lower phase can be assessed ) to model potential CCC response to various solvent mixes.

The user should aim to achieve a partition coefficient of between 0.5 and 1.5 and should then choose an appropriate mix and examine the affect of pH on this partitioning.   

The effect of pH on partitioning can on occasions be very considerable Slide 16.

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